畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (2): 273-278.doi: 10.11843/j.issn.0366-6964.2015.02.014

• 预防兽医 • 上一篇    下一篇

鸡包涵体肝炎病原检测及病理学观察

王婉,王彦红,刘伊,金文杰,石火英*   

  1. (扬州大学兽医学院病理教研室,禽类预防医学教育部重点实验室,扬州 225009)
  • 收稿日期:2014-06-17 出版日期:2015-02-23 发布日期:2015-02-23
  • 通讯作者: 石火英,E-mail:hyshi@yzu.edu.cn
  • 作者简介:王婉(1989-),女,甘肃白银人,硕士生,主要从事禽传染病方面的研究,E-mail:wangjiawanyun@126.com
  • 基金资助:

    国家自然科学基金(31172300);江苏省高校自然科学重大项目(12KJA230002);高等学校博士学科点专项科研基金(博导类)(20133250110002);江苏高校优势学科建设工程资助项目(PAPD);江苏省动物重要疫病与人兽共患病防控协同创新中心

Pathogenic Detection and Pathological Observation of Inclusion Body Hepatitis in Chicken

WANG Wan,WANG Yan-hong,LIU Yi,JIN Wen-jie,SHI Huo-ying*   

  1. (Key Lab for Avian Preventive Medicine of Ministry of Education,College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China)
  • Received:2014-06-17 Online:2015-02-23 Published:2015-02-23

摘要:

取临床疑似鸡包涵体肝炎病例的肝组织,接种SPF鸡胚获取尿囊液,对获取的尿囊液进行PCR扩增和基因测序分析,成功分离并鉴定出1株鸡包涵体肝炎病毒,同时对送检病例采样进行病理组织学观察。将病死鸡肝组织研磨后,经尿囊腔接种9日龄的SPF鸡胚,用获得的尿囊液提取病毒DNA,成功获得DNA模板。根据已发表的Ⅰ群禽腺病毒hexon全基因的保守区域设计并合成1对引物,用这对引物对病毒的DNA模板进行PCR扩增,结果扩增出与目的基因大小一致的片段。病理学观察见肝组织淤血,细胞核浓缩,发生坏死,核内出现包涵体,伴有脂肪变性。测序分析表明,分离株与Ⅰ群禽腺病毒中血清4型相似性为98.6%,与其他血清型的相似性为68.7%~96.9%,与Ⅱ群禽腺病毒的鸡出血性肠炎病毒和Ⅲ群禽腺病毒的减蛋综合征病毒的相似性为44.6%,最终确定分离病毒为Ⅰ群禽腺病毒,为进一步鉴定和分离Ⅰ群禽腺病毒提供了依据。

Abstract:

One strain of the inclusion body hepatitis virus (IBHV) was isolated and identified based on pathological changes,PCR and sequence analysis in sick chicken.The DNA of the IBHV was extracted from allantoic fluid obtained from SPF egg,which was inoculated with the sick chicken liver.The primers were designed based on the conserved hexon sequence of avian adenovirus.The PCR product of avian adenovirus was amplified and sequenced.The fatty degeneration,karyopyknosis,cell necrosis and basophilic inclusion bodies were observed in liver tissue from sick chicken.The sequencing results indicated that the virus shared 98.6% identity with FAV-4 in group Ⅰ and 68.7%-96.9% identity with other serotypes in group Ⅰ,and 44.6% identity with hemorrhagic enteritis virus in group Ⅱ and egg drop syndrom virus in group Ⅲ.These results confirmed that the isolated virus is a member of fowl adenovirus groups,and can cause chicken inclusion body hepatitis.Our research provides the basis for isolation and identification of group Ⅰ avian adenovirus.

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